The effect of human chorionic gonadotropin (hCG) on sperm fertilization rate following IVF

Background: The positive effect of human chorionic gonadotropin (hCG) on the sperm capacitation, presence of hCG in fallopian tube secretions and semen and presence of hCG receptor on rat sperm has been confirmed, therefore in this study the effect of hCG on the fertilization rate following IVF was investigated.Methods: Given the results of our previous study on the hCG effect on the sperm motility, in this study, hCG with concentrations of 25 and 100 ng/ml was used in T6 medium for sperm capacitation with incubation time of 120 minutes. Then, 6 hours following IVF, the rate of fertilization was calculated with counting two pronuclei (2PN).Result: The rate of fertilization increased significantly (P≤0.05) in hCG experimental group with concentration of 25 ng/ml compared to 100 ng/ml and it tended to be increased compared to control group. Also, the rate of fertilization decreased significantly (P≤0.05) in hCG experimental group with concentration of 100 ng/ml compared with control group.Conclusion: The results of this study demonstrated that the effect of hCG hormone on the fertilization rate is dose-dependent, so that hCG with concentration of 25 ng/ml lead to an increase in sperm fertilization rate.

کلیدواژه ها:

Fertilization rate ، In vitro fertilization (IVF) ، Sperm motility ، Human chorionic gonadotropin (hCG)