A successful live birth 3 years after vitrification of oocytes and sperms

Background: This study is based on a report that 3 years after vitrification of both oocytes and sperms in a couple diagnosed with infertility, a live birth occurred using their oocytes and sperms. Methods: In 2011, a couple, female aged 37 and male aged 35 years old, referred to Shiraz Infertility Center. They were diagnosed with 18 months of infertility due to male factor. After ovarian stimulation with standard protocol, 12 oocytes were retrieved; but due to male partner inability to provide sperm sample, all oocytes got cryopreserved using a vitrification protocol. The male partner, diagnosed with varicocele at the time, underwent successful varicocelectomy operation. After varicocelectomy, his sperm samples got harvested and frozen as the female partner was unexpectedly diagnosed with MS at that point and wasn’t ready for pregnancy.Result: After remission of her MS, the frozen sperm samples and oocytes were thawed and ICSI process began. 83% out of 12 thawed oocytes survived and got inseminated by ICSI. 6 out of 10 oocytes got fertilized and developed into cleavage staged embryos. 3 of the embryos, that were in their 3rd day were transferred to the female partner uterus. At 7 weeks of gestation, a sonographic exam revealed one gestational sac with an alive fetus, and at 39 weeks of gestation, a normal female baby, weighing 3000 gr. was delivered.Conclusion: Our case report suggests that cryopreservation of oocytes, sperms and embryos, individually or combined, can be used as a preferred solution to reserve fertility ability in patients require time for pregnancy preparations.

کلیدواژه ها:

Oocyte cryopreservation ، Pregnancy ، Sperm freezing ، Vitrification ، Live birth