The effect of troxerutin administration during prepubertal period on total number of spermatogenic cells in type I diabetic rats
- سال انتشار: 1396
- محل انتشار: سومین کنگره بین‌المللی تولیدمثل
- کد COI اختصاصی: ISERB03_179
- زبان مقاله: انگلیسی
- تعداد مشاهده: 495
نویسندگان
Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
چکیده
Background: It is believed that the production of reactive oxygen species and development of oxidative stress is one of the major events for testis damage in diabetic patients. Troxerutin (TX) is a trihydroxyethylated derivative of the natural bioflavonoid rutins and commonly known as vitamin P4, possess well-proven antioxidant activities but there is no report to investigate whether troxerutin has a supportive effect on spermatogenesis process in diabetic peoples.Methods: Fifty prepubertal (2 weeks old) male wistar rats were divided into five groups including: Control, Diabetic (DM), TX, DM+Insulin and DM+TX. Type I diabetes was induced by a single dose of streptozotocin (IP, 55 mg/kg). Treatment groups received troxerutin (OG, 150 mg/kg/day) and insulin (SC, 4-6 IU/day) for 4 consecutive weeks. Finally, animals euthanized and then left testes were fixed in buffered formalin 10%. The samples were processed by standard paraffin embedding and serially sectioned at 20 μm thickness. Twenty to twenty-five sections per animal were selected through systematic random sampling and stained by H&E. Total numbers of spermatogenic cells were estimated by optical dissector and stereo-investigator system using an unbiased counting frame.Result: Stereological studies showed that diabetes decreased total number of all spermatogenic cells significantly (pConclusion: Based on our results, it can be concluded that administration of TX is a suitable protective strategy for side effect of diabetes in testis of prepubertal diabetic males.کلیدواژه ها
Diabetes, P4, Prepubertal, Spermatogenic cells, Troxerutinاطلاعات بیشتر در مورد COI
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