The effect of troxerutin administration during pubertal period on total volume of germinal epithelium of seminiferous tubules in type I diabetic rats
- سال انتشار: 1396
- محل انتشار: سومین کنگره بین‌المللی تولیدمثل
- کد COI اختصاصی: ISERB03_178
- زبان مقاله: انگلیسی
- تعداد مشاهده: 439
نویسندگان
Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
چکیده
Background: Basal generation of ROS is essential for male reproductive function and identify as an independent biomarker of male infertility in diabetic patients. Troxerutin or vitamin P4 is a flavonoid that can be found in tea, coffee and cereal and has many pharmacological properties such as anti-inflammatory and antioxidant activities but there is no data about its supportive effects on fertility of adult male diabetic peoples.Methods: Fifty pubertal (8 weeks old) male wistar rats were divided into five groups including: Control, Diabetic (DM), TX, DM+Insulin and DM+TX. Type I diabetes were induced by a single dose of streptozotocin (IP, 55 mg/kg). Treatment groups received troxerutin (OG, 150 mg/kg/day) and insulin (SC, 4-6 IU/day) for 4 consecutive weeks. Finally, animals euthanized and then left testes were fixed in buffered formalin 10%. The samples were processed by standard paraffin embedding and serially sectioned at 20 μm thickness. Twenty to twenty-five sections per animal were selected through systematic random sampling and stained by H&E. A total volume of testis was calculated by Scherle s method and volume density of germinal epithelium (VGE) was estimated by point- counting and Cavalieri method by stereo-investigator system using an unbiased study.Result: Stereolocal results indicated that diabetes decreased VGE significantly (pConclusion: Therefore, it can be concluded that administration of TX is a suitable protective strategy to inhibit side effect of diabetes on seminiferous tubules of pubertal diabetic males.کلیدواژه ها
Diabetes, Germinal epithelium, P4, Pubertal, Testis, Troxerutinاطلاعات بیشتر در مورد COI
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