Comparison between reactive oxygen species levels and antioxidant capacity of follicular fluid from polycystic ovary syndrome and healthy women undergoing assisted reproductive techniques
- سال انتشار: 1396
- محل انتشار: سومین کنگره بین‌المللی تولیدمثل
- کد COI اختصاصی: ISERB03_153
- زبان مقاله: انگلیسی
- تعداد مشاهده: 488
نویسندگان
Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده
Background: Polycystic ovary syndrome (PCOS) is a multifaceted disorder with a pathogenic pathway that is not completely understood yet. Apart from hormonal derangements, changes in antioxidants enzymes activity and oxidative stress may be having a role in this syndrome complications. Therefore, in this study, we investigated follicular fluid (FF) reactive oxygen species (ROS) levels and activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in PCOS and healthy women.Methods: Twenty women with PCOS were compared with regard to follicular fluid oxidant and antioxidant status with 20 age- and body mass index (BMI)-matched healthy controls. Freshly follicular fluid aspirated from dominant follicles on the day of oocyte retrieval. ROS levels were evaluated by Luminol-based enhanced Chemiluminescence assay and were represented in relative light unit (RLU). GSH-Px and SOD activities (IU/mL) were measured by specific colorimetric methods. In this study, the comparison between the means of two unrelated groups was undertaken using independent t-test. All statistical analysis performed using SPSS software v. 24.0.Result: RLU in FF of PCOS women was higher than normal(514.12±88.69 vs. 117.14±13.22). Also, SOD activity and GSH-Px activity were lower than the controls significantly (1.62±0.05 vs. 1.73±0.02 and 28.82±4.75 vs. 51.48±12.01 respectively).Conclusion: Although underlying mechanisms have not been fully explicated yet, it becomes evident that oxidative stress holds a significant role in the pathogenesis of PCOS. In fact, PCOS can be considered as a purely oxidative state, where the body antioxidants cannot scavenge the excessive production of free radicals.کلیدواژه ها
Follicular fluid, Glutathione peroxidase, Reactive oxygen species, Superoxide dismutase, Polycystic ovary syndromeاطلاعات بیشتر در مورد COI
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