Histological evaluation of testis in male mice following torsion-detorsion of testis by mesenchymal cells and crocin
- سال انتشار: 1396
- محل انتشار: سومین کنگره بین‌المللی تولیدمثل
- کد COI اختصاصی: ISERB03_150
- زبان مقاله: انگلیسی
- تعداد مشاهده: 516
نویسندگان
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Department of Radiology, Urmia University of Medical Sciences, Iran
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
چکیده
Background: In this study, the effect of mesenchymal cells (MSCs) and crocin (Cr) on the histological structure of testes following torsion-detorsion were evaluated.Methods: MSCs were isolated from bone marrow of the mice. 24 male mice were randomly divided into 4 groups and torsion-detorsion (TD) was performed at 720 degrees’ counter clock wise for half an hour in all groups, and injection of MSCs was performed in rete-testis. 1- Control group (C): In this group PBS was injected in left testes. 2- MSCs group: MSCs (5×105 in 10μL) was injected into the left testis. 3- Cr group: All mice received Cr 200mg/kg (IP). 4- MSCs+Cr group: All mice received MSCs along with Crocin. After 35 days, the testis of the mice was removed and fixed in a 10% buffered formalin and after tissue processing, paraffin sections were stained with H&E for histological investigations.Result: The results showed that the mean number of active Sertoli cells and Leydig cells in group MSCs + Cr was significantly increased in comparison to other groups (PConclusion: This histomorphometric study showed that the MSCs along with Cr were more effective in amelioration of testicular torsion damage.کلیدواژه ها
Crocin, Mice, Torsion, Mesenchymاطلاعات بیشتر در مورد COI
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