Investigation of polyunsaturated fatty acids omega-3 effects on In-Vitro fertilization rate in the female mice with polycystic ovarian syndrome

Background: Polycystic ovarian syndrome is the most common endocrine disorder among women in reproductive age. These women are in high risk for early onset of cardiovascular diseases. The aim of this study was investigating the effects of omega-3 fatty acids supplement on In-Vitro fertilization rate in the female mice with polycystic ovarian syndrome.Methods: 42 female mice were randomly divided into 6 groups as described below according to the treatment they received; N-C (Normal Control: Without PCO + Sesame oil treatment), C (Control: PCO + No treatment), N-O-300 (Without PCO + O3 300mg/kg per day), P-O-300 (PCO + O3 300mg/kg per day), N-O-600 (Without PCO + O3 600mg/kg per day), P-O-600 (PCO + O3 600mg/kg per day). In this study, intramuscular injection of estradiol valerate was used to make the samples infertile. The treatment period was 56 days. Histological analysis, biochemical parameters evaluated on the samples of ovary and hormone therapy tests were evaluated on the samples with in-vitro fertilization (IVF).Result: Results indicated that embryo formation in the two-cell and blastocyst levels increased in N-O-300, N-O-600 groups in comparison with other groups and also percentage of zygote formation increased in P-O-300, P-O-600 groups in comparison with C group.Conclusion: Omega-3 fatty acids had beneficial effects on embryo formation rate in women with polycystic ovary syndrome.

کلیدواژه ها:

Female mice ، In-vitro fertilization ، Omega3 ، Ovary ، Polycystic ovarian syndrome ، Polyunsaturated fatty acids