Comparison between different High Bisphenol-S dosage detrimental effects on IVF outcome in female mice

Background: Endocrine disrupting chemicals (EDCs) is a substance that alters function(s) of theendocrine system and consequently causes adverse health effects in an intact organism, or its progeny, or (sub) populations (WHO/ IPCS, 2002). Bisphenol-A (BPA) is one of the main EDCs which is found in pharmaceutical agents, pesticides and Plastics. Because of endocrine and oxidative stress effect, now a day, companies use BPA substitute in their product. Bisphenol-S, a dominant BPA replacement especially in cans and bottle, use in BPA-Free product in European countries. However, new investigation revealed 80 and 200 mM of BPS induce protein damage in human peripheral mononuclear cells. In our study, BPS high and normal dosage toxic effect was investigated on oocyte fertilization and blastocyst rate.Methods: In this study, 50 mature healthy female mice were housed for one week before investigation. All mice were divided to five groups randomly. Mice were administrated normal saline in control group. Sham group was administrated ethanol (concentration of the ethanol in the media was 0.1%). Three dosages of BPS were administrated 10 μg/kg, 50 μg/kg and 100 μg/kg in different groups. Sub cutaneous (SC) injection was used in 21day for decrease liver first past effect.Result: In all BPS administrated groups, long term BPS absorption induced reduction in oocyte fertility rate and blastocyst formation. Statistical analyses showed significant difference between control, sham and BPS absorbed groups. All high dosages of BPS (esp. 50 μg/kg & 100 μg/kg) have dominant destructive effect on oocyte fertilization and blastocyst formation rate.Conclusion: According to previous studies, BPS as a substitute play good role in plastic industry because of lesser EDCs effect than BPA. But there is a concern about High dose absorption of Bisphenol family. Our study elucidated that High dose consumption of BPS have destructive effect on In Vitro Fertilization (IVF). This is a new concept in infertility treatment because too many plastic devises were used in IVF procedure concerning increase in plastics compound consume in daily life.

کلیدواژه ها:

Bisphenol-S ، IVF ، Mice