Toll-like receptor signaling pathways in granulosa cells of infertile women suffering from Polycystic Ovarian Syndrome

سال انتشار: 1396
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 407

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شناسه ملی سند علمی:

ISERB03_048

تاریخ نمایه سازی: 11 خرداد 1397

چکیده مقاله:

Background: Growing evidences indicate polycystic ovary syndrome (PCOS) is associated with innate immune responses and systemic inflammation. The aim of this study was to evaluate the expression of Toll -Like Receptors (TLR) signaling pathways downstream in patient with PCOS.Methods: Twenty infertile PCOS patients and 20 normal women with male factor infertility as control group were enrolled. Granulosa cells was isolated using Ficol density gradient centrifugation. The expression of TLRs and signaling pathway molecules in granulosa cells were evaluated using real-time polymerase chain reaction. Macrophage migration inhibitory factor (MIF), Interleukin-6 (IL6) and IL8 proteins in follicular fluid samples was measured by ELISA.Result: The mean relative expression of TLR1-6, TLR8, TLR9, myeloid differentiation primary response gene 88 (MYD88), Toll-interleukin 1 receptor (TIR) domain-containing adapter protein (TIRAP), TIR-domain-containing adapter-inducing interferon-β (TRIF), TRIF-related adaptor molecule (TRAM) , Nuclear factor-kappa B (NF-kB), interferon regulatory transcription factor 3 (IRF3), interferon B (INFB), MIF, IL6 and IL8 genes was significantly higher in patients with PCOS in compare to normal women (PConclusion: Our findings suggested that TLRs gene overexpression and subsequently increased inflammatory markers such as MIF, IL6 and IL8 may be responsible for impaired folliculogenesis and oocyte maturation in PCOS.

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نویسندگان

Reza Gholamnezhadjafar

Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Seyed Abdolvahab Taghavi

Fertility and Infertility Research Center, Hormozgan University of Medical Sciences, Bandarabbas, Iran

Elham Amirchaghmaghi

Department of Endocrinology and Female Infertility at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

Reza Salman Yazdi

Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran