Extraction of Dentine Matrix Proteins for Using in Dental Tissue Engineering

  • سال انتشار: 1388
  • محل انتشار: شانزدهمین کنفرانس مهندسی پزشکی ایران
  • کد COI اختصاصی: ICBME16_140
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 2070
دانلود فایل این مقاله

نویسندگان

F.S Tabatabaei

Department of Dental Materials, Faculty of Dentistry Tehran University of Medical Sciences

T.S Jafarzadeh

Department of Dental Materials, Faculty o

J Ai

Research center for Medical and Technology in Medical Science , Tehran University of Medical Sciences Department of Tissue engineering , Faculty of Advance Technology , Tehran University of Medical Sciences

A.H Tavakkoli

Iranian Tissue Bank Research &Preparation Center , Imam khomeini Hospital , Tehran University of Medical Sciences

چکیده

The dentine matrix is a reservoir of growth factors and cytokines, sequestered during dentin genesis. Previous studies have demonstrated that EDTA solubilised dentine matrix components show morphogenetic activity and can induce dentino genesis . The aim of this issue was to extract dentin matrix protein in order to study lastly, their effects on cultured endometrial adult stem cells. Materials & Methods: ex vivo teeth were obtained and the pulps , enamel and cementum were removed dentin was ground and placed in 10% (EDTA) and 5 M PMSF ( Ph7.4) for 10 days. Superior liquids were put in dialysis sacs prior to electrophoresis. Result: protein fractions from dentin were separated by electrophoresis confirmed that the individual peaks demonstrated heterogeneity in their protein content . Conclusion: Reciprocal epitheliomesenchymal interactions control the terminal differentiation of odonatoblasts, Since these interactions are matrix mediated , attempts have been made to understand the nature and mechanism (s) of cell – matrix interactions . In this study , we have extracted dentin matrix proteins . Afterward , we would study the effect of these proteins on the differentiation of endometrial adult stem cells to odontoblast cells.

کلیدواژه ها

dentine matrex proteins , EDTA Demineralization, dental tissue engineering

مقالات مرتبط جدید

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.