Tahereh Shokrian - Department of Agronomy and Plant Breeding, Aburaihan Campus, University of Tehran, Tehran, Iran
Seyed Ahmad Sadat Noori - Department of Agronomy and Plant Breeding, Aburaihan Campus, University of Tehran, Tehran, Iran
Ghorban Ali Nematzadeh - Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari agricultural sciences and natural resources university, Sari, Iran
Seyed Mohammad Alavi - Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari agricultural sciences and natural resources university, Sari, Iran

Trachyspermum copticum (Apiaceae) is an annual plant which grows in Iran. The fruits of T. copticum (Ajwain) traditionally were used as diuretic, carminative, and antihelmentic. Some biological effects of Ajwain such as antiviral, antifungal and antioxidant activities have been confirmed. The objective of the present investigation was toevaluate the antibacterial activity of extracts of callus and seed and essential oil of Ajwain against some bacterial strains (Pseudomonas viridiflava, Pseudomonas syringae pv. syringae and Escherichia coli). The extracts and essential oil were prepared and the antibacterial activity was evaluated via growth inhibitory zone assay using disc diffusion agar technique. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was measured by micro broth dilution assay. The results revealed no significant effect for callus extract, however, the effect of seed extract and essential oil on tested bacterial strains was statistically significant. The greatest impact was observed for essential oil and inhibition halo diameter was reported 28.5 mm for P. syringae pv. syringae, MIC and MBC were measured 1.56 and 3.12% v/v, respectively.

antibacterial activity, Trachyspermum copticum, callus