Effect of KLK2 nsSNPs on prostate related disease

  • سال انتشار: 1396
  • محل انتشار: هفتمین همایش بیوانفورماتیک ایران
  • کد COI اختصاصی: IBIS07_022
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 513
دانلود فایل این مقاله

نویسندگان

F Toghraei Semiromi

Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, ۴۷۴۱۶-۹۵۴۴۷, Babolsar, Iran

E Moudi

Department of Urology, Babol University of Medical Sciences, Babol, ۴۷۷۴۵-۴۷۱۷۶, Iran

A Hosseinzadeh Colagar

چکیده

Kallikrein2 codes serine protease enzyme that cleaves pro-PSA to active PSA which has an important role in liberation of sperm and fluidity of semen by shattering of Semenogelin and Fibronectin. Any polymorphism of this gene could effect on serine protease activity [1] , which means they may associate with prostate cancer. In this study 7 nsSNPs were chosen which H65Y, and G214E are in active site; D207N, and S228A are in substrate binding site, and R250W that its GMAF is more than 1%, R24W in cleavage site and W38R. The influence of these substitutions on function and stability of protein and being deleterious were predicted by Polyphen2.0, Provean, Imutant3.0 and SIFT [2-5] . All 7 nsSNPs were predicted as affected on protein functions. The PolyPhen 2.0 predicts that D207N and S228A are possibly damaging, G214E, R24W, W38R and H65Y are probably damaging and R250W is benign. Also D207N, G214E, R24W, W38R and H65Y are deleterious while S228A, and R250W are neutral. Unlike the other nsSNPs, the H65Y may increase the stability. Among intended nsSNPs, D207N has destructive effect on this enzyme, while R250W may has less destruction. Change of hK2 enzyme function by mentioned nsSNPs could associate with some prostate diseases

کلیدواژه ها

Deleterious nsSNPs; KLK2; infertility; in-silico

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.