p najafi - Protein Research Centre, Shahid Beheshti University, Tehran, G. C., Velenjak, Iran
h Kouchakzadeh - Protein Research Centre, Shahid Beheshti University, Tehran, G. C., Velenjak, Iran

Cancer diagnosis in its early stage of progress, can enhance the efficiency of treatment by use of the conventional therapy methods. In order to reach fast and effective diagnosis, numerous developments had been made on utilizing contrast agents such as magnetic nanoparticles. Non-biocompatibility of such a nanoparticle made a big challenge in front of their usage. Efficient coverage by biomolecules such as albumin can be a solution to overcome this problem. Herein, albumin coated magnetic nanoparticles were prepared and the process was optimized using the RSM. Under optimum conditions of 2.28 mg magnetic nanoparticles and pH 9.21 loading of 7.76% obtained with the particles size of 170 ±12 nm and zeta-potential of -36.4 ±2.3 mV. Physicochemical and paramagnetic properties of nanoparticles offer them as a conventional tool for cancer diagnosis after verification of their efficiency in vitro and in vivo.

Early cancer diagnosis; Optimal production; Contrast agents; Superparamagnetic iron oxide nanoparticles; Albumin nanoparticles