The effects of cytotoxic dose of progesterone on caspase 9 activity level in colon cancer (ht29) cells

The HT-29 cell line is the most widely used colorectal adenocarcinoma cell line with epithelial morphology.Progesterone is the most primitive hormone among all reproductive hormones. Caspases are a family of endoproteases that provide critical links in cell regulatory networks controlling inflammation and cell death. Caspase-9 is a member of the cysteine proteases involved in cytokine and apoptosis processing. Cytotoxic concentrations (0.01mg/ml) of progesterone was used in our study. HT29 cells were purchased from National Cell Bank of Iran . Cells were grown and incubated in standard situation. Then, cells were sub-cultured into 75cm2 flasks, 96-well plates or 6-well plates. Activity level of caspase 9 was evaluated using calorimetric assay (405nm) through microplate reader. Analyses were conducted using the SPSS 20 and ANOVA. Our results indicated that exposure to 0.01mg/ml of progesterone led to significant increase in caspase 9 activity compared to control cells. In our study we found that exposure of colon cancer cells to cytotoxic dose of progesterone led to increased activity of caspase 9 enzyme indicating that apoptosis occurred through internal pathway in which caspase 9 is activated. Human colon cancer cells are also affected by sex steroid hormones. Sex steroid hormone including progestins have pivotal role in proliferation of colon cancer cells. The studies also have indicated that apoptosis in several types of cancer cells is associated with caspase 9 activity. In our study we found that exposure of colon cancer cells to cytotoxic dose of progesterone led to increased activity of caspase 9 enzyme indicating that apoptosis occurred through internal pathway in which caspase 9 is activated.

کلیدواژه ها:

Colon Cancer ، Cancer Symptoms ، Cell and Cancer ، Cancer Treatment and Management ، Drugs and Cancer