Investigating the effect of zinc oxide nanoparticles synthesized by green chemistry method on liver cancer cells

Plant-mediated nanoparticles biosynthesis is a green chemistry method, which connects nanotechnology with plants. Main advantage of using green methods is avoiding the use of toxic reducing agents, majority of which are highly reactive and are unsafe to the environment. Here we synthesized zinc oxide (ZnO) nanoparticles in Rubia tinctorum extract and evaluate its cytotoxicity on breast cancer cell line. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability assay was performed on HepG2 liver cancer cell line. The cells were incubated 24 hours with different concentration of ZnO nanoparticles including (31.25, 62.5, 125 and 250 μg/ml) compared to HUVEC normal cells as a control.The results showed that Zn O nanoparticles kill cancer cells in a concentration-dependent manner. The IC50 values for cytotoxicity of Zn O nanoparticles synthesized in Rubia tinctorum extract were 32 and 60 μg/ml for HepG2 liver cancer and HUVEC normal cells, respectively. Results of this study showed that Zn O nanoparticles synthesized in Rubia tinctorum extract are able to inhibit cancer cells in lower concentrations than normal cells, which indicated Zn O efficacy in killing cancer cells

کلیدواژه ها:

Gastric Cancer ، Cancer Prevention ، Cell and Cancer ، Cancer Treatment and Management ، Drugs and Cancer