Evaluation of the toxicity of silver nanoparticles on cancer cells (hepg2) compared to normal cells (l929)

سال انتشار: 1396
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 305

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شناسه ملی سند علمی:

NASTARANCANSER03_072

تاریخ نمایه سازی: 7 اسفند 1396

چکیده مقاله:

Nanotechnology refers to the identification materials in the range of 1-100 nm. In this size, the physicochemical and biological properties of the materials are unusual and unconventional, and new applications of these materials can be seen. This technology is growing rapidly in various fields and Nanomaterials are widely used in medicine, pharmaceuticals, electronics and cosmetic. Research showsthat 56% of the world s total nanoparticles are dedicated to silver nanoparticles. The nanoparticles are resistant to heat and have a high stability. Silver nanoparticles are synthesized using various methodssuch as chemical, physical and biological methods (through fungi and bacteria). In this study, the cytotoxic effect of silver nanoparticles has been evaluated against cancer and normal cells. MTT is oneof the common methods used to evaluate the toxicity of various materials. This method is usually evaluated at different times doses of substance. In this method, the survival rate of the treated cells atdifferent concentrations and times is evaluated relative to the untreated cells. First the cells are seeded in 96 well plate then were exposed with different doses of the nanoparticle for 24, 48 and 72 h. MTTand following that DMSO solutions were used to evaluate toxicity of nanoparticle. Morphological observations were also performed to evaluate the effect of the silver nanoparticle on the appearance ofthe cells using the Invert microscope. Time and concentration are the two most effective factors in the toxicity of materials against of cells. The obtained results of this study showed that the both factorshave significant effects on viability of cells. As observed, with increasing concentration and time of treatment, cell survival significantly decreased and IC50 was about 5, 4.5 and 3 μg/ml 24, 48 and 72hafter treatment. Reductions in survival were observed at 48% post-treatment period from about 64% to 3% in the studied concentrations. Cytotoxic results showed that the amount of inhibition of cancercells and normal cells after 72h exposures was about 0.15 and 3 μg/ml respectively , which indicates higher toxicity of the nanoparticle on liver cancer cells compared with normal cells. Morphologicalchanges such as reduction in volume, size, wrinkling of the cell and the formation of cytoplasmic sprouts were recorded using an invert microscope. The results showed that the silver armor was able toinhibit cancer cells in lower concentrations than normal cells, which confirms the effect of the nanoparticles effectiveness on inhibition of cancer cells in the living tissue

کلیدواژه ها:

Cancer Prevention ، Cell and Cancer ، Solid Tumors ، Cancer Treatment and Management ، Drugs and Cancer

نویسندگان

Sahar Hajebi

Department Of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Masoud Homayooni Tabrizi

Department Of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Mahboobeh Nakhaei Moghaddam

Department Of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran