Binding Affinity of Cationic Gemini Surfactants to Insulin: Effect of the Spacer Length
- سال انتشار: 1395
- محل انتشار: چهاردهمین همایش بیوشیمی فیزیک ایران
- کد COI اختصاصی: CBC14_008
- زبان مقاله: انگلیسی
- تعداد مشاهده: 623
نویسندگان
Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Faculty of Biological Sciences, Alzahra University, Tehran, Iran
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
Department of Resin and Additives, Institute for Color Science and Technology, Tehran, Iran
چکیده
Gemini surfactants are composed of two surfactants that are chemically linked together by a spacer attheir hydrophilic head groups.Because of their applications in academic and industrial fields, Geminisurfactants have drawn great attention of scientists of various communities, since 1991.Some of Geminishave reported as food stabilizers [1]. Others are claimed to be potential candidates for gene delivery [2].The interaction of Gemini surfactants with nucleic acids have also studied widely. However, theinteraction of Geminis with proteins has been the subject of only recent studies[3]. In this research, theinteraction of two cationic Gemini surfactants with insulin is investigated by fluorescence spectroscopy.The two Gemini surfactants differ in their spacer length (C6, C4). Titration of insulin solution with theGemini dispersion (C6 or C4) was performed and then, the fluorescence spectra recorded. The binding ofboth surfactants to the protein was evidencedby quenching the intrinsic fluorescence of protein uponaddition of the surfactants.The Stern-volmer plots were applied to derive the binding parameters such asKa and n. Results demonstrate that the longer the spacer length of the surfactant, the lower the bindingaffinity to insulin is. The number of binding sites for surfactant per protein molecule also decreases, as thespacer length increases.کلیدواژه ها
Cationic Gemini Surfactant; fluorescence spectroscopy; insulin; interactionمقالات مرتبط جدید
- بررسی اثرات انتشار امواج لرزه ای در سازههای قاب خمشی دسته شده و هگزاگرید دارای پارامتر سختی یکسان
- شناسایی آزمایشگاهی ترک طولی تراورس بتنی پیش تنیده با استفاده از تست مودال تجربی
- Investigation of Transmission Loss and Absorptioncoefficient of the PU Composite Panels forConstruction Application
- Comprehensive Analysis of the Effect of Polyisobutyl-ene (PIB) Molecular Weight on Damping Properties of Butyl-Based Constrained Layer Damping Systems
- REVIEWING THE OBERST BEAM METHOD AND ITS AP-PLICATION IN CONSTRAINED LAYER DAMPING FOR AUTOMOTIVE NOISE, VIBRATION, AND HARSHNESS (NVH) CONTROL
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.