Study Of Methylation Status In PTEN Gene In Iranian Breast Cancer Patient

PTEN tumor suppressor gene encodes a protein that plays a role in increasing the level ofphosphorylation. PTEN gene mutation effects of tumorigenic properties is unknown. PTEN gene isdisabled by genetic and epigenetic mechanisms such as hypermethylation. CpG islandhypermethylation is another route to tumour suppressor gene inactivation however, the literatureregarding PTEN hypermethylation in cancer is controversial. To evaluate the frequency ofmethylation of PTEN gene in patients with Breast cancer, the methylation specific PCR using exon 7(MS-PCR) PCR and restriction enzyme (PER) was dependent. After examining 50 patients withBreast cancer by MS-PCR and PER hypermethylation in 42% and 48%, respectively, were found.Background check pathologic tissue samples of cancer patients showed that the PTEN geneinactivation may be performed in the early stages of cancer and epigenetic factors influencing theprogression of cancer. We show that methylation of the PTEN CpG island is a rare event in cancercell lines and that apparent methylation most likely originates from homologous regions of thePTENP1 pseudogene promoter.