Cytotoxic Effects Of Gold Nanoparticles Coated With Amino Acid Aspartate And Glutamate On PC12 Class Cells

Nowadays, the gold nanoparticles in cancer therapy will provide an entirely new technology. Theaim of this study was to investigate the toxic effects of gold nanoparticles coated with amino acids ofAspartate and Glutamate on PC12 category cells.In this empirical study after PC12 cell categoryculture as in vitro these cells are divided into 9 groups and separately for 48 hours exposed tovarying concentrations of gold nanoparticles (1, 2 and 4 μM gold nanoparticles, gold nanoparticlescoated with amino acids Aspartate and Glutamate). At last, the effect of nanoparticle cytotoxicityusing MTT and LDH release from the cells was examined. The phase contrast microscopy was used toevaluate the cellular morphology. At end, the level of ROS in the cells using fluorescent probes 2′, 7′-Dichlorofluorescein diacetate was measured.the average diameter of the gold nanoparticles in thecase of uncoated and coated with amino acids Aspartate and Glutamate was calculated at size of10±0.2, 24±0.4 and 27±0.4 nm, respectively. The most damaging effect of the studied cells byconcentration of 5 μM of gold nanoparticle coated with amino acid Aspartate occurred at a rate of47%, when compared to the group treated with bare gold nanoparticles and gold nanoparticlescoated with Glutamate had a statistically significant difference (p≤ 0/05). The results of this studyindicate that the majority of cell death of incubated cells with uncoated and coated goldnanoparticles is as apoptosis The results showed that gold nanoparticle coated induces cell deathand also gold nanoparticle coated with amino acid can destroy cancer cells through apoptosis.