Mohammad Hosein Izadpanah - Department Of Biology, Islamic Azad University, Damghan Branch, Damghan, Iran
Mohammad Reza Abbaszadegan - Division Of euman Genetics, Immunology Research Center Avicenna Research Institute,Mashhad University Of MedicalSciences(MUMS),Mashhad,Iran
Mohammad Mahdi Forghanifard - Department Of Biology, Islamic Azad University, Damghan Branch, Damghan, Iran

Esophageal squamous cell carcinoma (ESCC) is the sixth and ninth most common cancer betweenmen and women worldwide, respectively.TWIST, a member of the beLe transcription factor familyplays a key role in the specification and differentiation of the tissues It may function as amultifunctional proto-oncogene during tumorigenesis and progression of solid tumors. OCT-4 (alsoknown as POU5F1) is a key regulator of self-renewal in embryonic stem cells. the expression of suchgenes is probably correlated with tumorigenesis and may have an effect on some aspects of tumorbehavior, such as tumor recurrence or resistance to therapies. Our aim in this study was to evaluateregulatory role of TWIST1 on OCT4 in ESCC cell line KYSE30. mRNA extraction and cDNA synthesiswere performed for the ectopic expressed and normal KYSE30 cell lines. the probable role of TWIST1over expression in regulation of CSC marker OCT4 was assessed in esophageal squamous cellcarcinoma (KYSE-30) using comparative Real-time PCR method. After ectopic expression of TWIST 1in KYSE30 cells, we have observed significant overexpression of TWIST1 nearly 10 fold. This inducedexpression of TWIST1 caused significant upregulation of OCT4 in KYSE-30 cells. TWIST1 as amastermind in morphogenesis plays an important role in metastasis and invasion of tumor cells.Furthermore, our results show that TWIST1 over expression can be correlated with cancer stem cellmarker OCT4 as well.TWIST1 may plays critical regulatory role in gene expression of CSC markerOCT-4 which is involved in self renewal process of ESCC. These results suggest new role for TWIST1in CSC biology.