Partial purification of Glutathione S transferase from Gammarus using ammonium sulfate precipitation
- سال انتشار: 1396
- محل انتشار: اولین کنفرانس بین المللی علوم دریایی و جوی: محیط زیست، انرژی های تجدید پذیر
- کد COI اختصاصی: MASERE01_027
- زبان مقاله: انگلیسی
- تعداد مشاهده: 538
نویسندگان
Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
Department of Microbiology, Faculty of medicine, Babol University of Medical Sciences, Babol, Iran
چکیده
Glutathione S-transferase (GST) is a multifunctional enzyme that is thought to plays a role in the start of xenobiotic detoxification process. This enzyme catalyzes the reaction between xenobiotic compounds and the -SH groups. This reaction leads to reduced biological activity and increased solubility. So, the resulting compounds are more polar and therefore easier to remove. Gammarus species are sensitive to a wide range of contaminant sand are suitable for the detection of environmental pollutants therefore they can be used as a model organism in the study of environmental pollution. The partial purification of GST was the main purpose of this study. In order to reduce the volume, concentration and purification of proteins, various precipitation methods can be used. Salting out with appropriate concentration of ammonium sulfate, which is a highly soluble salt, separate protein with different solubility. In our study solid ammonium sulfate with final concentration of 60% and 90%was added to Gammarus homogeneous and the mixture was stirred until dissolving of the salt. The suspension was centrifuged at 18000×g for 20 minutes, then the supernatant was dialyzed overnight at 4 C against 100 mM phosphate buffer, pH=7.Protein concentration was estimated by Bradford method and GST activity was assayed by Habig method. Protein concentration was decreased in supernatant of both 60%and 90% saturations. Enzyme activity had a 3-fold decrease in 90%, button the contrary, a4-fold increase was observed in enzyme activity in 60% saturated solution. In conclusion, the findings suggest precipitation with ammonium sulfate affords a procedure for concentrating as well as purifying the enzyme.کلیدواژه ها
Gammarus, Glutathione S transfrase, Ammonium sulfate Precipitation, Purificationمقالات مرتبط جدید
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.