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Protocol of optimization tomato tissue culture for the micropropagation and recombinant protein production (Feron genotype)

عنوان مقاله: Protocol of optimization tomato tissue culture for the micropropagation and recombinant protein production (Feron genotype)
شناسه ملی مقاله: AARC01_012
منتشر شده در اولین کنفرانس سالانه تحقیقات کشاورزی ایران در سال 1394
مشخصات نویسندگان مقاله:

Masomeh Nasiri Mogadam۱ - Graduated in agriculture biotechnology of advanced technology Kerman Graduate University
Mokhtar Jalali Javaran - Associate Professor of Plant Breeding, Faculty of Agriculture, Tarbiat Modarres University
Mehdi Mohammadi - Assistant Professor of Plant Breeding, Faculty of Agriculture, University of Kerman
Shahram Poor Seyedi - Associate Professor Institutes of Environmental Sciences of Technology Graduate University for Advanced Kerman

خلاصه مقاله:
For years, plants used for the production of recombinant proteins and as Molecular farming is known. However, the transformation of tomatoes is still not A common method and repeatable for all digits. In this study, we’ve optimized the tissue culture of tomato genotype (Feron genotype) with two cotyledon and hypocotyl explants in 6 different culture medium whit different hormones. In the meantime, culture medium supplemented whit benzyl amino purine (BAP) mg/L3 and Zeatin mg/L1 In addition whit 0.5 mg/L indole acetic acid (IAA) showed the highest regeneration rate. Interaction among Culture medium and explants on callus induction and regeneration were observed. Among the explants cotyledon was the best regeneration.

کلمات کلیدی:
tomatoes, optimize tissue culture, the role of hormones

صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/605339/