The Effect of Deep eutectic solvents on catalytic function and structure of Bovine Liver Catalase

سال انتشار: 1395
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 378

متن کامل این مقاله منتشر نشده است و فقط به صورت چکیده یا چکیده مبسوط در پایگاه موجود می باشد.
توضیح: معمولا کلیه مقالاتی که کمتر از ۵ صفحه باشند در پایگاه سیویلیکا اصل مقاله (فول تکست) محسوب نمی شوند و فقط کاربران عضو بدون کسر اعتبار می توانند فایل آنها را دریافت نمایند.

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

CHCONF03_072

تاریخ نمایه سازی: 8 اسفند 1395

چکیده مقاله:

Growing interest in using deep eutectic solvents (DES) as a nonconventional media in biotransformation reactions encouraged us to investigate the effect of aqueous Reline (choline chloride: urea) and Glyceline (choline chloride: glycerol) solutions, two common deep eutectic solvents, on function and structure of Bovine Liver Catalase (BLC). In this study UV-Visible, fluorescence and circular dichroism (CD) were used as spectroscopic methods. According to the results, in the presence of 100 mM glyceline solution, the binding affinity of the substrate to the enzyme increased which caused a decrease in Michaelis-Menten constant value (Km). Due to CD spectroscopy spectra, this might be related to changes in secondary structure of protein. Regarding activity measurements, enzyme remained nearly 70% active in this concentration of glyceline solution; however, in the similar concentration of reline, in spite of the lower tendency of the substrate to bind to the protein, enzyme remained 80% active. With increasing DES concentration, enzyme activity started to decrease and in concentrated solutions mainly inactivated. Surprisingly it stayed nearly 40% active in choline chloride solution which is the common ion species in reline and glyceline solvents. It is proposed that the instructive chaotropic nature of choline cation might put stop on reducing trend of activity in concentrated choline chloride solutions but this instructive effect is lost in concentrated deep eutectic solutions. The presence of deep eutectic solvent components in biological media increases the possibility of forming these solvents in human cells as an activity adjuster for this important enzyme in its different operation conditions.

نویسندگان

Roohollah Ghobadi

Department of Chemistry, Kharazmi University, Tehran, Iran

Ali Reza Harifi-Mood

Department of Chemistry, Kharazmi University, Tehran, Iran

Adeleh Divsalar

Department of Biological Sciences, Kharazmi University, Tehran, Iran