Glassy carbon electrode incorporating Prussian blue nanoparticles for voltammetric determination of D-penicillamine

Penicillamine (PA) (SH-C(CH3)2-CH(NH2)-COOH, 2-amino-3-mercapto-3-methylbutanoic acid, β,β- dimethylcysteine, 3-mercaptovaline) is derived from hydrolytic degradation of penicillin,which has many therapeutic applications. This compound is a strong chelating agent and can react with the majority of heavy metal ions. The outstanding metal-binding capability is reflected in the pharmaceutical importance of PA [1]. The typical therapeutic dose administered to humansis in the range of 0.5–2.0 g daily. Increasing the amount of penicillamine can cause rashes early in treatment. It can also cause loss of appetite, nausea, abdominal pain, and loss of the sense of taste. While the D-enantiomer of PA is therapeutic, the L-enantiomer is highly toxic; since itpossesses the same configuration as L-amino acids (which are the constituents of proteins) and can interfere with amino acid metabolism [2]. This work describes the electrocatalytic properties of a glassy carbon electrode (GCE) modified with poly(o-phenylenediamine) (PoPD)/Prussian blue (PB) nanoparticles (NPs) for the electrocatalytic oxidation of D-Penicillamine (D-PA). Thestepwise fabrication process of PBNPs-modified PoPD/GCE was characterized by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy. The mechanism and kinetics of the catalytic oxidation reaction of D-PA were monitored by cyclic voltammetry andchronoamperometry. Some important advantages such as simple preparation, fast response, good stability and reproducibility of the sensor for the voltammetric determination of D-PA were achieved.

کلیدواژه ها:

D-penicillamine ، Prussian blue nanoparticles ، Poly(o-phenylenediamine) ، Glassy carbon electrode