Sanaz Akbarzadeh - Department of Textile Engineering, Science and Research Branch, Islamic Azad University, Yazd, Iran
Navid Nasirizadeh - Department of Textile Engineering, Science and Research Branch, Islamic Azad University, Yazd, Iran

Streptomycin, the earliest of the aminoglycosides, that is an antimicrobial organic base produced by Streptomyces griseous and has found widespread use in both human and veterinary medicine[1,2]. In agriculture it is used to control bacterial and fungal diseases of selected fruits, vegetables, seeds, specialized field crops, ornamental crops and in ornamental ponds and aquaria to control algae [3]. Although antibiotic residues in food have no direct toxic effect on humanhealth, numerous adverse effects can range from fever and nausea to major allergic reactionsincluding photodermatitis and anaphylaxis [4]. Therefore introducing a selective and sensitive method for accurate determination of antibiotics is crucial. . Recently, electrochemicaltechniques have been applied to determine antibiotics because they offer an opportunity for portable, rapid and cheap methodologies.Purpose of research In this work, the electrocatalytic oxidation of Streptomycin on a screen printed electrode modified with graphenoxide, oracet blue and silver nanoparticles (GOBAgNPs-SPE) has been studied. The (OBAgNPs-SPE) shows highly catalytic activity toward Streptomycin electrooxidation. The results indicated that Streptomycin peak potential at GOBAgNPs−SPE shifted for 340 mV to negative values as compared with bare electrode surface. Fig.1 shows theelectrocatalytic effect of GOBAgNPs-SPE toward oxidation of STR. The kinetic parameters,such as the electron transfer coefficient, α, and the standard heterogeneous rate constant, k', for oxidation of Streptomycin at the GOBAgNPs-SPE were determined. The amperometric detectionof Streptomycin was carried out at 400 mV in a 0.1 mol L-1 phosphate buffer solution (pH 7.0) resulting in two linear response ranges of 0.4 to 240.0 nM and 240.0 to 720.0 nM and the detection limit of 0.17 nM. ConclusionThe GOBAgNPs−SPE can decrease the oxidation peak potentials of Streptomycin. Therefore,determination of the analyte (Streptomycin) is possible without any interference. Moreover, the GOBAgNPs−SPE was used to determine Streptomycin in real samples with satisfactory results

Graphenoxide; Oracet blue; Streptomycin; Silver Nanoparticle; Screen printed electrode