Assessment of tissue distribution and subcellular localization of miR-302 and miR-21 by means of in situ hybridization technique
- سال انتشار: 1391
- محل انتشار: مجله سلول و تحقیقات مولکولی، دوره: 4، شماره: 2
- کد COI اختصاصی: JR_JCMR-4-2_007
- زبان مقاله: فارسی
- تعداد مشاهده: 531
نویسندگان
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Pathology laboratory, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran
Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
چکیده
MicroRNAs (miRNAs) are a group of short non-coding RNAs implicated in numerous fundamental cellular processes, and their disregulations have been linked to several pathologic conditions, mainly cancers.Determining tissue distribution of miRNAs is a prerequisite for understanding their exact functions during development, tissue homeostasis and abnormality. In situ hybridization is a powerful technique to delineate the sub-cellular localization and tissue distribution patterns of mRNAs as well as miRNAs. Due to the important role of miRNAs in tumorigenesis, we optimized an ISH technique for detection of two well-known miRNAs(miR-302 and miR-21) in formalin-fixed paraffin-embedded (FFPE) tumor samples along with a pluripotentembryonal carcinoma cell line, NTERA-2 (NT2). After fixation of cells on slides/sectioning of FFPE blocks,proteinase K digestion, probe concentration, antibody development and light sensitive color reaction were optimized for both the FFPE samples and cell line. Signals for U6 snRNA, as an internal control, were detectedin the nuclei of the cells. MiR-21 and miR-302 expression was detected in the cytoplasm of FFPE samples ofseminoma carcinoma and in NT2 cell line, respectively. In this study, we optimized ISH for miRNA detectionin FFPE samples and NT2 cell lineکلیدواژه ها
in situ hybridization, microRNA, miR-302, miR-21مقالات مرتبط جدید
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.