Identifying thrips (Insecta: Thysanoptera) using DNA Barcodes

  • سال انتشار: 1389
  • محل انتشار: مجله سلول و تحقیقات مولکولی، دوره: 2، شماره: 1
  • کد COI اختصاصی: JR_JCMR-2-1_005
  • زبان مقاله: فارسی
  • تعداد مشاهده: 619
دانلود فایل این مقاله

نویسندگان

Javad Karimi

Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Mahnaz Hassani-Kakhki

Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

Mehdi Modarres Awal

Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

چکیده

Thrips tabaci Lindeman is an extensively distributed pest insect in many areas that affects plants through direct feeding and at the same time, it makes damage as a vector of different viruses. As a basic first step tocontrol pests is authentic identification, but the inability to determine morphological characters of thrips species makes this process very difficult. For creating an identification key for T. tabaci, an economicallyimportant species present in Iran, four individuals were selected from four different sites of Mashhad and the vicinity, each as a separate population. The method was based on nucleotide sequencing analysis of themitochondrial cytochrome C oxidase I (COI) gene. Phylogenetic analyses conducted by the neighbor-joiningmethod yielded almost identical phylogenetic reconstructions of trees that separated thrips based on thegeographic origin. Molecular data indicate that different thrips species are located in distinct groups. These results show that molecular keys can be a useful method to provide much-needed information on thrips identification for pest management officers and quarantine purposes

کلیدواژه ها

Thrips tabaci, mitochondrial DNA, barcoding, phylogenetic tree, molecular identification

مقالات مرتبط جدید

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.