Detection of human cytomegalovirus by using PCR in immunocompromised persons

سال انتشار: 1395
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 697

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شناسه ملی سند علمی:

MPHBS01_039

تاریخ نمایه سازی: 22 آبان 1395

چکیده مقاله:

Introduction: Human cytomegalovirus (HCMV) is a member of herpesviruses family and recently has been recognized as an immunodeficient virus, it's so important because most of people have it. Because of this reason and clinical protests of infected patients to this virus caused by reducing levels of their immune, the aim of this study was to detect the CMV virus quickly and check the accuracy of the two methods of Real-Time PCR and cytochemical in immunocompromised people for HCMV defects. Materials and methods: 100 blood samples were collected from immunocompromised persons in Tehran. Real-Time PCR and cytochemical staining were used as the two methods for detecting HCMV. The first method was based on the TaqMan and the second one, on characterizing cell morphological identification. Results: The positive PCR test results were 36% between 40 cases and 21% between 35 cases for cytochemical. It was also found that the maximum prevalence occurred in the summer with 34% and the minimum with 22% that belongs to the autumn. However, a significant relationship didn't observe between the prevalence of HCMV and the season. Conclusion: The results showed that, with an accurate Real-Time PCR, we can recognize HCMV immunocompromised people. But cytochemical stain method can be used along with this method for the final confirmation and for a better diagnosis. It was also found that, the highest rate of HCMV infection, either latent or active form, is related to the fall season, but this association was not significant.

نویسندگان

Seyed Bashir Mirtajani

Department of biothechnology, Faculty of Basic Sciences, Lahijan Branch, Islamic Azad University (IAU), Lahijan, Iran.

Saman Ayoubi

Allergy and Asthma Immunology Research Center, Tehran University of Medical sciences, Tehran, Iran.

Jafar Aghajani

Mycobacterium Research Center, NRITLD, Shahid Beheshti of Medical Sciences, Tehran, Iran