Cytotoxic Effect and Cell Attachment of IL-12 Antimicrobial Peptide on A549 Epithelial Cells

Antimicrobial peptides, as host defense peptides are one of the major part of the innateimmune response found among all classes of life. These peptides are potent, broad spectrumantibiotics which demonstrate potential as novel therapeutic agents. The majority ofconventional antibiotics it appears as though antimicrobial peptides may also have the abilityto enhance immunity by functioning as immunomodulators. IL-12 with the sequence ofILGTILGLLKGL-NH2 is an antimicrobial peptide derived from the venom of the neotropicalsocial wasp (Agelaia pallipes pallipes). The aim of this study was to evaluate ROS and cellattachment of A549 cell line treated by IL-12 at 12 Âμg/ml and 50 Âμg/ml. To examine theamount of ROS, the cell was incubated for 24 and 48 hours in the presence of the peptide.After adding DCF-DA as fluorescence probe, the amount of ROS was measured by flowcytometry. Results showed that at 50 Âμg/ml of the peptide, ROS was 26.7% and 84.7% over24 h and 48 h- treatment, respectively; whereas, the amount of ROS value in control cellswas 0.6%. Cell attachment experiments using crystal violet staining revealed that at 12Âμg/ml and 50 Âμg/ml of the IL-12 peptide, the cell attachment values were 101% and 102%,whilst, this value for control cell was 26.1%. Our results suggest that the peptide possessesthe binding ability to the cells leading to cell attachment. Furthermore, treatment of A549 cellwith IL-12 peptide increased the ROS

کلیدواژه ها:

Cytotoxic Effect ، Cell Attachment ، IL-12 Peptide ، A549 cell line ، ROS