Differential Expression of linc-ROR in Tumor&margin of Tumor Samples of Breast Cancer

Breast cancer is the most common invasive cancer in females worldwide. It accounts for 16%of all female cancers and 22.9% of invasive cancers in women. 18.2% of all cancer deathsworldwide, including both males and females, are from breast cancer. The long non-codingRNAs (lncRNAs) are RNA molecules over 200 nt in length with little protein-codingpotential.Recent studies have revealed that lncRNAs are involved in regulation of multiplebiological processes, including development, differentiation and carcinogenesis .ThelncRNA linc-ROR was upregulated in breast tumor samples, and ectopic overexpression oflinc-ROR in immortalized human mammary epithelial cells induced an epithelial-tomesenchymaltransition (EMT) program. Breast tissue specimens were obtained from theEmam KHomeini Hospital. Samples were frozen in liquid nitrogen immediately after surgicalremoval. Total RNA was extracted from breast tissue specimens using the Trizol reagent.The cDNA was generated with random primers using the Reverse Transcription System withTakara kit. RT-PCR analysis. To evaluate the clinical relevance of linc-ROR in humanmammary carcinomas, the linc-ROR expression level in clinical specimens was assessed byquantitative RT-PCR. In a collection of 22 pairs of randomly chosen samples of breast cancerpatients, we detected that the linc-ROR level was significantly higher in cancerous tissuescompared with the adjacent non-cancerous tissues (Normal). linc-ROR expressed a higherlevel in breast cancer cell lines. These results suggested that increased level of linc-RORmay be a feature involved in breast tumorigenesis and cancer progression. This studyidentifies the lncRNA linc-ROR as a novel potential oncogene in breast cancer throughinduction of an EMT program. linc-ROR as a potent target for new therapies for the highlyaggressive and malignant breast cancers in clinical practice.

کلیدواژه ها:

RT-PCR ، Breast cancer ، linc-ROR ، LncRNA ، Tumor