The Effect of 7-Methoxy-4,5-Dihydro-2H-Benzo[g]Indazole on Induction of Apoptosis and Monocyte/macrophageDifferentiation in Human Chronic Myelogenous Leukemia (K562) Cells

سال انتشار: 1394
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 424

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شناسه ملی سند علمی:

NASTARANCANSER01_045

تاریخ نمایه سازی: 26 شهریور 1395

چکیده مقاله:

There are some reports on the anticancer effects of Pyrazole derivative. The goal of this studywas to examine the anti-proliferative and apoptosis-inducing characteristics of this PyrazoleTricyclic derivative. In this experimental trial, K562 cell line was cultured in the presence of100 to 0.001 mg/ml concentrations of the Pyrazole Tricyclic derivative for 24, 48 and 72 hour.Anti-proliferative and cytotoxic effects of Pyrazole Tricyclic derivative were measured bytrypan blue exclusion test and MTT assay, respectively. Apoptosis-inducing effects weremeasured by apoptotic DNA fragmentation test. Wright-Giemsa staining was used fordetermining the effects of Pyrazole Tricyclic derivative, and latex phagocytic assay was usedfor evaluating the phagocytic potential of the differentiated cells. The results showed that theK562 cells viability were sensitive to Pyrazole Tricyclic derivative. The highest apoptosis washappened in 100μg/ml at 48 hours. Encountering cells with that material caused to induceapoptosis in relation with time and concentration. This finding indicated that PyrazoleTricyclic derivative has just an apoptotic-inducing effect without any differentiation.

نویسندگان

Mina Akrami Mardagh

Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran

Nahideh Afzal ahangharan

Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran

Noruz Delirezh

Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran