Effect of the Medium Composition on Formation ofAlpha Amylase by Bacillus licheniformis GMAF438149

A new medium was developed to induce high level of α- amylase from Bacilluslicheniformis GMAF438149.The strain of Bacillus licheniformis was grown on the presence of different carbon , nitrogen and metal ions. The high level α-amylase has been produced when potato starch and xylose were added to medium. Meat extract ,as an organic nitrogen source, had affected on enzyme yield , while inorganic sources were not found to be effective. Calcium chloride increased activity of the produced enzyme ;however, a slight activity was observed by increase of oxygen concentration. The optimum temperature of Bacillus licheniformis GMAF 438149 for α–amylase production was determined 39°c and the optimum pH was 6.8 . Enzyme was stable at elevated temperature ,and was unstable at varied pH values .Inhibitory effects of Zn and Cu were observed on α-amylase production