Vitrification affects the expression of matrix metalloproteinases and their tissue inhibitors of mouse ovarian tissue
- سال انتشار: 1395
- محل انتشار: International Journal of Reproductive BioMedicine، دوره: 14، شماره: 3
- کد COI اختصاصی: JR_IJRM-14-3_003
- زبان مقاله: انگلیسی
- تعداد مشاهده: 609
نویسندگان
School of Biology, Damghan University, Damghan, Iran.
School of Biology, Damghan University, Damghan, Iran.
School of Biology, Damghan University, Damghan, Iran.. Institute of Biological Sciences, Damghan University, Damghan, Iran.
School of Biology, Damghan University, Damghan, Iran.. Institute of Biological Sciences, Damghan University, Damghan, Iran.
چکیده
Background: One of the most major obstacles of ovarian tissue vitrification is suboptimal developmental competence of follicles. Matrix metalloproteinases 2 (MMP-2) and 9 (MMP-9) and their tissue inhibitors TIMP-1 and TIMP-2 are involved in the remodeling of the extracellular matrix in the ovaries.Objective: This study aimed to evaluate the expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 genes in the preantral follicles derived from vitrified mouse ovaries.Materials and Methods: In this experimental study, the gene expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the isolated preantral follicles derived from fresh and vitrified ovaries of 14-16 days old female mice through real time qRT-PCR was evaluated. Developmental parameters, including survival rate, growth, antrum formation and metaphase II oocytes were also analyzed.Results: The developmental parameters of fresh preantral follicles were significantly higher than vitrified preantral follicles. The TIMP-1 and MMP-9 expression levels showed no differences between fresh and vitrified preantral follicles (p=0.22, p=0.11 respectively). By contrast, TIMP-2 expression significantly decreased (p=0.00) and MMP-2 expression increased significantly (p=0.00) in vitrified preantral follicles compared with to fresh ones.Conclusion: Changes in expression of MMP-2 and TIMP-2 after ovarian tissues vitrification is partially correlated with decrease in follicle developmentکلیدواژه ها
Vitrification, Pre antral follicles, Matrix metalloproteinases, Mouseاطلاعات بیشتر در مورد COI
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