Protective effect of methanolic extract of Berberis integerrima Bunge. root on carbon tetrachloride-induced testicular injury in Wistar rats
محل انتشار: مجله طب تولید مثل ایران، دوره: 14، شماره: 2
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 446
فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJRM-14-2_009
تاریخ نمایه سازی: 16 شهریور 1395
چکیده مقاله:
Background: Tissue protective effect of compounds with antioxidant properties has been demonstrated. The alkaloids found in barberry root are considered as antioxidants.Objective: According to barberry protective effects in different tissues, in this study, the protective effect of Berberis integerrima Bge. root (MEBIR) was evaluatedagainst CCl4-induced testicular damages in Wistar rats. Materials and Methods: 40 mature male rats were randomly divided into 5 groups:1: Normal control, 2: Sham: received CCl4 diluted in olive oil (50% v/v; 1ml/kg bw), intraperitoneally, twice a week for 4 weeks, 3 and 4: Sham rats treated withMEBIR (250 and 500 mg/kg bw) for 28 days, 5: Sham rats treated with silymarin (50 mg/kg bw) for 28 days. After 28 days, serum testosterone level, absolute testis weight, catalase activity, malondialdehyde level, and histological parameters wereinvestigated. Results: In the treated rats with MEBIR (250 and 500 mg/kg bw) or silymarin (50 mg/kg bw), there was a significant increase in the absolute testis weight, testosteronelevel, seminiferous tubules diameter (p<0.001), thickness of the epithelium, tubule differentiation index (p<0.001), spermiogenesis index (p<0.001), the activity ofcatalase, and a significant decrease in interstitial tissue thickness (p<0.001) and malondialdehyde level in comparison with CCl4-treated group. The effect of the MEBIR at dose of 500 mg/kg bw is more than that of the standard drug, silymarin (50 mg/kg bw). Conclusion: From the results, it is suggested that the protective effects of MEBIR is possibly due to antioxidant effects of its bioactive compounds
کلیدواژه ها:
نویسندگان
Fereshteh Rafiee
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
Vahid Nejati
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
Reza Heidari
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran
Hossein Ashraf
Department of Biology, Faculty of Science, Urmia University, Urmia, Iran