Derivation of ES-like cell from neonatal mouse testis cells in autologous sertoli cells co-culture system

  • سال انتشار: 1393
  • محل انتشار: International Journal of Reproductive BioMedicine، دوره: 12، شماره: 1
  • کد COI اختصاصی: JR_IJRM-12-1_006
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 646
دانلود فایل این مقاله

نویسندگان

Mohammad Hossein Asadi

Department of Anatomical Sciences, Faculty of Medical Sciences, Baqiyatallah University of Medical Sciences, Tehran, Iran

Setareh Javanmardi

Department of Anatomical Sciences, Faculty of Medical Sciences, Baqiyatallah University of Medical Sciences, Tehran, Iran

Mansoureh Movahedin

Department of Anatomical Sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran

چکیده

Background: Spermatogonial stem cell (SSC) is a self-renewing population of male adult stem cell. SSCs have a differentiation potential which are similar to embryonic stem cells. These Embryonic stem like (ES-like) cells can be a potential source for pluripotent cells for stem cell-based therapy.Objective: This study presents an economical and simple co-culture system for pluripotent stem cells generation from neonatal mouse testisMaterials and Methods: Isolated testicular cells were cultured in DMEM/F12. Characteristics of the isolated cells and obtained ES-like cell were immune-cytochemically confirmed by examining the presence of PLZF, vimentin, Oct4 and Nanog protein. Expression of the pluripotency and germ-cell specific genes was analyzed by qPCR in derived ES-like colony and SSCs respectively.Results: The experiment results indicated that our method of obtaining pluripotent ES-like cells from spermatogonial cells (SCs) is simpler than the described methods. ES-like cells were immunopositive for pluripotency markers. ES-like cell qPCR results indicated significant increase in pluripotency genes expression and significant decrease in germ cell-specific genes expression.Conclusion: The results indicated that ES-like cell with pluripotency characteristic were generated from freshly isolated spermatogonial cells. The pluripotent stem cells provide a cellular reservoir usable for regenerative medicine instead of embryonic stem cells.

کلیدواژه ها

Spermatogonial stem cells, Embryonic stem like cell, Pluripotent stem cells, Co-culture

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.