Biodegradation of high concentrations of phenol by baker’s yeast in anaerobic sequencing batch reactor

  • سال انتشار: 1394
  • محل انتشار: مجله مدیریت و مهندسی بهداشت محیط، دوره: 2، شماره: 2
  • کد COI اختصاصی: JR_EHEM-2-2_006
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 611
دانلود فایل این مقاله

نویسندگان

Ali Asghar Najafpoor

Associate Professor, Health Sciences Research Center, Department of Environmental Health Engineering, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran

Abbas Sadeghi

Assistant Professor, Health Sciences Research Center, Department of Environmental Health Engineering, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran

Hossein Alidadi

Associate Professor, Health Sciences Research Center, Department of Environmental Health Engineering, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran

Mojtaba Davoudi

Assistant Professor, Health Sciences Research Center, Department of Environmental Health Engineering, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran

چکیده

Background: Phenol, as a pure substance, is used in many fields because of its disinfectant, germicidal, local anesthetic, and peptizing properties. Aqueous solutions of phenol are produced as waste in industries and discharged into the environment. Therefore, elevated concentrations of phenol may be found in air or water because of industrial discharge or the use of phenolic products.Method: The strains of Saccharomyces cerevisiae used in this project were natural strains previously purchased from Razavy company. They were grown at 30°C on Petri plates containing yeast extract glucose (YGC) and then purified by being spread onto new plates, and isolated colonies were obtained. These colonies provided the basis of selection. Prepared strains were applied in anaerobic sequencing batch reactors (ASBRs) as first seed. The experiment conditions were optimized using response surface methodology (RSM). After the determined runs were performed using Design-Expert software, data were analyzed using mentioned software as well.Results: This study evaluated the capability of baker’s yeast to remove phenol in high concentrations. The tested strains showed excellent tolerance to phenol toxicity at concentrations up to 6100 mg/L. Study of the batch degradation process showed that the phenol removal rate could exceed 99.9% in 24 hours at a concentration of 1000 mg/L. The results showed catechol is the first intermediate product of phenol degradation. In survey results of the Design–Expert software, R2 and Adeq precision were 0.97 and 25.65, respectively.Conclusion: The results demonstrated that ASBR performs robustly under variable influent concentrations of inhibitory compounds. The high removal performance despite the high phenol concentration may be a result of reactor operating strategies. Based on the progressive increase of inlet phenol concentration, allowing for an enhanced biomass acclimation in a short time, results at the microbiological levels showed that the increase of phenol concentration was accompanied by a decrease in the microbial community and a progressive selection of the most adapted phenotypes.

کلیدواژه ها

Yeast, Phenol, ASBR, Biotransformation, Synthetic solutions, Design of experiments software

مقالات مرتبط جدید

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.