Shiva Sadat Shayegan-Salek - Ph.D. candidate, Division of Environmental Science and Engineering, Faculty of Engineering, National University of Singapore, Block EA #۰۳-۱۲, ۹ Engineering Drive ۱, Singapore۱۱۷۵۷۶.
Wun Jern Ng - Ph.D. candidate, Division of Environmental Science and Engineering, Faculty of Engineering, National University of Singapore, Block EA #۰۳-۱۲, ۹ Engineering Drive ۱, Singapore۱۱۷۵۷۶.
Jianzhong He - Ph.D. candidate, Division of Environmental Science and Engineering, Faculty of Engineering, National University of Singapore, Block EA #۰۳-۱۲, ۹ Engineering Drive ۱, Singapore۱۱۷۵۷۶.

This study demonstrated acidogenic sludge (pH 5.2) could produce hydrogen and also granulate in an anaerobic sequencing batch reactor (ASBR) fed with synthetic wastewater containing glucose with 6.66h hydraulic retention time (HRT) at ambient temperatures. Optimization, enrichment, and stability of the acidogenic granular sludge have been investigated at a constant loading rate of 25 g-glucose/ (L.d). Results showed that hydrogen in the biogas increased from 15% to 48% by subjecting the biomass to a combination of heat-treatment, acidic pH, and carbon source limitation. The maximum hydrogen, yield, and production rate was 73%, 2.5 molH2/mol glucose, and 0.34 molH2/d, respectively. Microbial analysis indicated that enrichment by granulation was successful and microbial diversity changed significantly after treatments. The ASBR was operated for 445 days. The hydrogen producing granules were characterized. A typical matured granule was 1.7mm in diameter with an average of 43 m/h settling velocity. Moreover, as morphological analysis demonstrated, the inner and outer surface of the granules was comprised the same types of bacteria and hence had non-layered structure. A hydrogen producing granule had multiple cracks on the surface. The acidified effluent comprised volatile fatty acids (VFA) and alcohols. The VFA comprised acetate (73%), butyrate (23%), propionate (1.5%), caproate (0.69%), valerate (0.58%).

alternative energy, hydrogen, granule, glucose, synthetic wastewater