The study of tellurite tolerance and its bioremoval by a halophilic actinobacterial strain, QS-2006

  • سال انتشار: 1386
  • محل انتشار: پنجمین کنگره بین المللی مهندسی شیمی
  • کد COI اختصاصی: ICHEC05_346
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 2040
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نویسندگان

Maryam Khoshnoodi

Dept. of Microbiology, Faculty of Biology, College of Sciences, University of Tehran, Iran.

Mohammad Ali Amoozegar

Dept. of Microbiology, Faculty of Biology, College of Sciences, University of Tehran, Iran.

Javad Hamedi

Dept. of Microbiology, Faculty of Biology, College of Sciences, University of Tehran, Iran.

چکیده

A Gram-positive moderately halophilic tellurite reducing actinobacterial strain was isolated from saline soil, and designated as strain QS-2006 .This strain showed high level tolerance to potassium tellurite up to 500 mM of tellurite. The liquid cultures of the strain QS-2006 reduce tellurite to its elemental and less toxic form. Tellurite removal was assayed by colorimetric method using diethyldithiocarbamate (DDTC). The results showed that at least 50% tellurite was removed in the presence of glucose or sucrose as a carbon source during 4 days. Effects of various factors including: temperature, mixing, various concentrations of salts and tellurite concentrations ware investigated. Mixing has an increasing effect on the removal rate. At concentration of 2mM by increasing the mixing from 0 to 220 rpm the removal rate reached up to 94%. At 34ºC and Te concentration of 0.4 mM and 2mM the removal is 69% and 92% respectively. Intracellular Te (0) deposits were showed by energy dispersive X -ray (EDX) analysis and transmission electron microscopic.

کلیدواژه ها

Bioremediation; Tellurite; Actinobacteria; Microbial Metal resistance; oxyanion

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