M Rahimpour - Protein Chemistry Unit, Biotechnology Research Center, Pateur Institute of Iran
A Eslami - Rabies Department, Pasteur Institute of Iran
M Zakeri - Protein Chemistry Unit, Biotechnology Research Center, Pateur Institute of Iran
A Fayaz - Rabies Department, Pasteur Institute of Iran

Prefractionation of comples protein mixtures is an efficient method in orther to increase the separation power of two - dimensional electrophoresis (2DE). Reverse Phase High Performance Liquid Chromatography (RP- HPLC) has been successfully utilized for prefractionation followed by 2DE. Here, optimization of a RP-HPLC prefractionation method of BHK cells lysate followed by 2DE is reported. A step gradient elution of cell lysate was improved to yield properly resolved fractions for subsequent analysis by 2DE.