Indirect in vitro regeneration of lentil (Lens culinaris Medik.)
- سال انتشار: 1391
- محل انتشار: دوفصلنامه اصلاح مولکولی گیاهان، دوره: 1، شماره: 1
- کد COI اختصاصی: JR_JPMB-1-1_006
- زبان مقاله: انگلیسی
- تعداد مشاهده: 407
نویسندگان
Collage of Agricultural, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, I.R. Iran
Department of Biotechnology and Plant Breeding, Collage of Agriculture, Ferdowsi University of Mashhad, P.O.Box 91775-1163, Mashhad, I.R. Iran
Department of Agricultural Biotechnology, Paiame noor University of Tehran, P.O. Box: 1599958515 Tehran,I.R. Iran
چکیده
Establishment of an efficient and reproducible regeneration protocol is one of the basic prerequisites forgenetic transformation of any crop plant. In vitro culture of lentil has proven to be difficult. In spite of anumber of reports on the regeneration of this plant, very few satisfying and reproducible protocol has yet beenreported. This study carried out for investigation of different hormone treatments and explants in order toestablish a reproducible protocol for indirect in vitro regeneration of the cultivar Gachsaran (commonlygrown in Iran). For this purpose, the effects of 13 different hormone treatments and 4 explants on callusinduction and regeneration were studied. Callus with the highest fresh and dry weight was produced onmodified Murashige and Skoog (MS) medium containing 1 mg/L α-naphthaleneacetic acid (NAA)and 1 mg/LZeatin (medium E). Among the explants, decapitated embryos attached to 1/4 of the cotyledon (DEAC)produced callus with the highest fresh and dry weights. In the regeneration stage, calli induced on mediasupplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) alone or in combination with other hormones didnot result in shooting or rooting responses. The highest shooting and rooting responses (75%) were observedfor callus induction medium E, using decapitated embryos with a quarter of the cotyledon as the explant.کلیدواژه ها
Callus, Phytohormones, Regeneration, Lentilمقالات مرتبط جدید
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.