Applications of insect cell culture and baculoviruse for recombinantprotein production: a review

سال انتشار: 1394
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 629

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شناسه ملی سند علمی:

ASECONF01_069

تاریخ نمایه سازی: 30 بهمن 1394

چکیده مقاله:

Insect cell cultures are widely used for the production of recombinant proteins, vaccines and viral pesticides. It has become a common expression system for both basic research and large-scale commercial applications. The baculoviruses expression system is based on the replacement of non-essential, viral gene, termed polyhedrin, with a gene of interest. The insertion of foreign DNA at the polyhedrin locus within the viral genome results in incorporation of the foreign DNA into progeny virus particles and subsequent high-level expression of therecombinant protein within eukaryotic insect cell lines. A main factor to the popularity of insect cell expression is the ability of insect cells to produce relatively large quantities eukaryotic proteins in a relatively short period of time. In this review, we focus on the cell line of insects and baculoviruses that used in BEV system and recombinant proteins production in this system

کلیدواژه ها:

Baculoviruse Expression Vectors system ، insect cell ، vaccin ، recombinant proteins

نویسندگان

Neda Hashemi

Young Researchers and Elite Club, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran.

Sayed Ali Mortazavi

Department of Food Science and Technology, Faculty of Agricultural Engineering, Ferdowsi University,Mashad, Iran

Samane Gazerani

Department of Food Science and Technology, Faculty of Agricultural Engineering,Islamic Azad University of Sabzevar, Iran.

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