Occurrence of Cucurbit aphid-borne yellows virus infection on melons in Sistan region

  • سال انتشار: 1394
  • محل انتشار: همایش بین المللی پژوهش های کاربردی در کشاورزی
  • کد COI اختصاصی: ICARA01_174
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 714
دانلود فایل این مقاله

نویسندگان

Javad Abkhoo

Institute of Plant Biotechnology, University of Zabol, Zabol, Iran

چکیده

Melon and watermelon are most important cucurbit crops in Sistan region and viral diseasesof cucurbit crops cause important economic losses in Sistan region. During summers of 2009,virus-like symptoms were noticed on cucurbit crops (watermelons and melons) grown in theSistan region of Iran. The symptoms were widespread and included initial chlorotic lesionsfollowed by yellowing of whole leaves and thickening of older leaves, suggesting thepresence of Cucurbit aphid-borne yellows virus. A survey was performed to investigate ayellowing viral disease in the major cucurbit-growing areas of Sistan region. Leaf samplesshowing typical symptoms (yellowing, thickening) were collected from melon plants ofseveral cucurbit-growing areas of Sistan region. All samples, were screened for the presenceof CABYV by double-antibody sandwich enzyme-linked immunosorbent assay (DASELISA).In order to confirm CABYV identification, total RNA extracts were obtained fromsamples that were positive using DAS-ELISA and reverse transcription-polymerase chainreactions (RT-PCR) were carried out. RT-PCR experiments yielded an expected 479-bpproduct similar to the fragment amplified with extracts from the reference isolate, RT-PCR.All samples were infected with Cucurbit aphid-borne yellows virus (CABYV). This is the first report of CABYV existence in Sistan region.

کلیدواژه ها

Cucumis melo, Citrullus lanatus, Iran, Cucurbit aphid-borne yellows virus

مقالات مرتبط جدید

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.