Preparation of Sodium Alginate nanoparticles containing Bovin Serum Albomin(BSA)

  • سال انتشار: 1393
  • محل انتشار: پنجمین کنگره بین المللی نانو و فناوری نانو (ICNN2014)
  • کد COI اختصاصی: ICNN05_267
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 509
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نویسندگان

M Rahimi Borumand

Department of chemistry Islamic Azad University, Shahr-e-rey branch,Tehran, Iran

A Zare Mirak Abadi

Razi Vaccine and Serum Research Institute, Karaj, Iran

N Mohammadpour Duonighi

Razi Vaccine and Serum Research Institute, Karaj, Iran

چکیده

Alginate, a natural biopolymer extracted from brown algae (kelp) has several advantages which have made it to be used as amatrix for excavation and delivery of a variety of biological agents.In present work, we were carry out a systematic study to prepare a new kind of biocompatible and biodegradable nanoparticlesfor loading Bovin Serum Albumin (BSA), and evaluating their potential as protein delivery system. In our study aseries of alginate nano-particles were prepared. Calcium chloride (CaCl2) was used as a cross-linking agent, cause of itscationic property, and poly-l-lysine was also used as polycationic agent to prepare matrix under ionic-gelation process. Theinfluence of some factors (BSA, polymer, CaCl2 concentration, homogenization rate and pH) in particles size, morphology,encapsulation efficiency (EE) and loading capacity (LC) were studied. FT-IR was used to confirm cross-linking processes ofpolymer with cationic agents and Brad Ford assay was also used to measure LC and EE. TEM and Z.SIZER were used formorphological and dimensional studies.It optimum condition the results were as follow; %EE and %LC were 89.25% and 89.23% consequently

کلیدواژه ها

Nanoparticles;Sodium Alginate;Cross-linking;Poly-L-Lysine;Encapsulation

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