Selective hydrolysis of fish oil using Rhizomucor miehei lipase immobilized on silica nanoparticles
- سال انتشار: 1393
- محل انتشار: پنجمین کنگره بین المللی نانو و فناوری نانو (ICNN2014)
- کد COI اختصاصی: ICNN05_042
- زبان مقاله: انگلیسی
- تعداد مشاهده: 330
نویسندگان
National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran,
National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran- Department of Chemistry, Khuzestan science and Research Branch, Islamic Azad University, Khuzestan, Iran
National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran- Department of Chemistry, University of Zanjaz, Zanjan, Iran
چکیده
we report immobilization of Rhizomucor miehei lipase (RML) by random and oriented methods. For thispurpose, silica nanoparticles (SBA-15 and MCM-41) were prepared, characterized and functionalized byglycidyloxypropyle trimethoxysilane (GPTMS). Random immobilization of RML was carried out on these supports viadifferent amino acid residue of enzyme in a mild condition. In order to perform oriented immobilization, the epoxyfunctionalized support was partially modified by introducing iminodiacetic acid groups fallowed by addition of Cu2+. Inthis type of immobilization the enzyme was quickly adsorbed on the support by the most accessible histidine group viametal chelate affinity interaction. Then irreversible covalent immobilization was carried out by the reaction between thenucleophilic residues of RML and the remaining epoxy groups of the support after 24h of incubation. The results showedhigher thermal stability for immobilized derivatives compared to the soluble RML. The ability of the immobilizedpreparations to selective hydrolysis of fish oil was studied at different conditions. The results demonstrated that all thederivatives distinguish between cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and cis-4,7,10,13,16,19-docosahexaenoic acid (DHA). According to effects of reaction parameters such as temperature, pH, time of hydrolysisprocess and amount of biocatalyst, increased discrimination of EPA and DHA.کلیدواژه ها
Rhizomucor miehei lipase, Oriented immobilization, Silica nanoparticles, SBA-15, MCM-41, Fish oilاطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.