Synthesis of Molecularly Imprinted Polymer Nano Particles as Sorbent in Matrix Solid-Phase Dispersion Extraction of Ochratoxin A in Coffee and Urine

For first time in this work a selective sample preparation technique using molecularly imprinted polymer(MIP) nanoparticles as a matrix of solid-phase dispersion extraction sorbents for the determination of ochratoxin A (OTA)is described. The MIP nanoparticles were successfully synthesized by precipitation polymerization applying ochratoxinA: methacrylic acid (MAA): trimethylolpropane trimethacrylate (TRIM) at mole ratio of 1:12:12. The morphology,binding, recognition behaviors of obtained particles were studied. The nanospheres with mean diameter values of 35 nm.Results from binding experiments demonstrated that the imprinted nanospheres with a 35 nm mean diameter had higherspecific affinity to ochratoxin A in contrast with non-imprinted particles. However, the binding performance of imprintednanospheres in real samples (coffee and urine) was estimated using high-performance liquid chromatography analysis.The recoveries of OTA from coffee spiked at 1, 25 and 50 ng g−1 and urine samples at 1, 25 and 50 ng mL−1 ranged from94.6 to 101.5%. The method exploits the high specificity of the MIP and the simplicity of solid-phase dispersion Methodwith MIP nano particles and shows considerable promise as it offers at least comparable or better sensitivity than the LCMS/MS methods.

کلیدواژه ها:

Molecularly Imprinted Polymer ، Nano Particles ، solid-phase dispersion extraction ، ochratoxin A