Anticancer and Antioxidant Properties of AgNPs@BSA

  • سال انتشار: 1393
  • محل انتشار: پانزدهمین کنگره ملی مهندسی شیمی ایران
  • کد COI اختصاصی: ICHEC15_540
  • زبان مقاله: انگلیسی
  • تعداد مشاهده: 895
دانلود فایل این مقاله

نویسندگان

M Azizi

Institute of Biochemistry and Biophysics (IBB), University of Tehran, Iran

H Ghourchian

Institute of Biochemistry and Biophysics (IBB), University of Tehran, Iran

F Yazdian

Facaulty of New Science and technology, University of Tehran, Iran

F Dashtestani

Institute of Biochemistry and Biophysics (IBB), University of Tehran, Iran

چکیده

This study examined the antioxidant and anticancer activities of silver nanoparticle coated with bovine serum albumin (AgNPs@BSA). Over the past few decades, there has been considerable interest in developing albumin nanoparticles as drug delivery devices. Bovine serum albumin (BSA) is an important endogenous antioxidants due to its potential of acting as Reactive oxygen species (ROS) Scavenger. On the other hand, toxicity of silver nanoparticles had been demonstrated on human cell lines. In the present study, silver nanoparticle were coated with bovine serum albumin (BSA) by a desolvation technique. The surface properties of the AgNPs@BSA were characterized by UV-Vis spectroscopy and Dynamic Light Scattering (DLS). Human breast cancer cells (MCF1 cells) were then cultured in the presence of the nanoparticles to evaluate the cytotoxicity of AgNPs@BSA by the MTT colorimetric technique. The antioxidant activities of AgNPs@BSA were evaluated in terms of their inhibition of autoxidation rate of pyrogallol as superoxide. The AgNPs@BSA formation was confirmed by DLS which showed nanoparticle size distribution from 775 nm to 789 nm and mean diameter of 740 nm. The effect of AgNPs@BSA on MCF1 exhibit a dose-dependent toxicity for the cell tested and the viability of MCF-7 decreased to 115 (LD11) at the concentration of 1 μg/ml. The IC50 value of antioxidant activities of AgNPs@BSA were 8 μg/ml which showed that AgNPs@BSA were good superoxide scavengers. In conclusion, our data show that AgNPs@BSA had antioxidant and anticancer activities in MCF-1 cells.

کلیدواژه ها

silver nanoparticles, Albumin, breast cancer cells, MCF-7

مقالات مرتبط جدید

اطلاعات بیشتر در مورد COI

COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.

کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.