Phenotypic and genotypic studies of extended spectrum betalactamase(ESBL) resistance among Salmonella isolates frompoultry sources in Iran

سال انتشار: 1391
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 365

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شناسه ملی سند علمی:

JR_IJVM-6-4_004

تاریخ نمایه سازی: 26 اردیبهشت 1394

چکیده مقاله:

BACKGROUND: Poultry and poultry products are among the major sources of Salmonella infections for humans. Increasing occurrence of antimicrobial resistance among Salmonellae has become a serious public health concern. The detection of extended spectrum b-lactamase (ESBL) producers among Salmonella spp. has increased in recent years. OBJECTIVES: The purpose of this study was to investigate the antibiotic resistance pattern ofSalmonella, and to understand whether ESBLs were present in Salmonella isolated from poultry farms and slaughterhouses from various parts of Iran. METHODS: A total of 314 isolates of Salmonellaspp., 272 of poultry and 42 from human origin, collected during winter 2005-2011 were characterized for antimicrobial resistance and the presence of ESBLgenes in this study. Phenotypic Disk diffusion method was performed for detection of antimicrobialsusceptibility against 16 antimicrobial agents according to the Clinical and Laboratory Standards Institute's recommendations (CLSI, 2005). To detect the presence of ESBL genes in 30 isolates out of 61 phenotypical resistant isolates, PCR amplification was used by employing specific primers for screening of theCTX-M and CMY groups, respectively. RESULTS: The highest resistance to ceforoxime in poultry and cefixime in human isolates was observed, and multidrug resistance (MDR) was seen with a maximum seven antimicrobial agents. The PCR detection of CTXM and CMY genes in all isolates including five phenotypically ESBLpositive isolates was negative. CONCLUSIONS: This study revealed the incidence of resistance to cephalosporins and thefrequency of MDR among Salmonella isolates from poultry farms in Iran. The prevalence of MDR Salmonella isolates from poultry are of particular concern as these strains can transmit to humans through the food chain.

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نویسندگان

M Rahmani

Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

S.M Peighambari

Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran