Evaluating expression alterations of two development related microRNAs, miR-134 and miR-187 in lung tumors.
- سال انتشار: 1393
- محل انتشار: اولین کنگره بین المللی و سیزدهمین کنگره ژنتیک ایران
- کد COI اختصاصی: CIGS13_0040
- زبان مقاله: انگلیسی
- تعداد مشاهده: 1007
نویسندگان
Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Department of Clinical Pathology, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
چکیده
Lung cancer is the leading cause of cancer-related death worldwide. Due to late diagnosis, the overall 5-year survival rate for NSCLC patients remains < 20%, thus, it is necessary to further elucidate the mechanisms involved in the tumorigenesis of lung cancer and discover novel diagnostics markers. microRNAs are a group of non-coding regulatory RNAs that play critical roles in biological processes during fetal development and tumorigenesis. Because of re-activation of developmental pathways in tumorigenesis, it is important to investigate role of development related microRNAs in lung tumors. Herein, we evaluated expression alterations of two development-related microRNAs, miR-187 and miR-134 in a matched case-control way. miR-134, an upregulated microRNA during lung development, induces cellular proliferation and epidermal-mesenchymal transition in lung tumor cells. miR-187 is down-regulated in developing lung. To investigate miRs expression patterns, 36 formalin-free paraffin-embedded (FFPE) samples of lung tumors along with matched non-tumor margin were obtained, RNA was extracted and after reverse transcription, Real-time qPCR was performed. Our data revealed that miR-134 was significantly up-regulated (p value: 0.0419) and miR-187 was significantlydown-regulated (p value: 0.0487) in tumor tissues. Up regulation of miR-134 is in agreement with its developmental status and shows its probable oncogenic roles. miR-187 down regulation indicates its possible tumor suppressing activity. According to these results, miR-187 and miR-134 could be used as potential lung cancer diagnostic biomarkers.کلیدواژه ها
lung cancer, miR-134, miR 187, development-related microRNAsمقالات مرتبط جدید
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.