Identification and Comparison of the Chloroplast DNA trnL-F of the Populus caspica Populations from the Hyrcanian Forests; North West of Iran
- سال انتشار: 1390
- محل انتشار: کنگره ملی ذخایر ژنتیکی و زیستی
- کد COI اختصاصی: IBRC01_043
- زبان مقاله: انگلیسی
- تعداد مشاهده: 747
نویسندگان
MSc student
Assco. Prof.; Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran
Assist. Prof.; Department of Biology, Faculty of Basic Sciences, University of Mazandaran
چکیده
The genus Populus is important member of Salicaceae.In the world have been identified more than 20 speciesof Salix and 50 species of populus. In addition havebeen described hundreds of varieties and hybrids.Since the trees are amplified in natural methods (sexualreproduction) and also in shoot cuttings method,because of hybridization between them, identificationis very difficult to identify. Populus caspica is native inHyrcanian forests and they usually grow in light-moistureis regions. The purpose of this study is indentification ofchloroplast genome based on the nucleotide sequenceof the non-coding regions cpDNA (trnL-F) in the P.caspica populations and comparison with other Populusin the NCBI gene bank. Two samples from seven sitesof P. caspica, were collected. Total genomic DNA wasextracted from frozen leaf tissues using of the Doyleand Doyle method. Polymerase chain reaction (PCR)performed by using of Pfu DNA polymerase andoligonucleotide primers UEUFF/UEUFR which designedby Taberlet et al. (1991). The chromatograms of the DNAsequencing results were processed and analyzed by theBlast software and were sequenced with other sequencesof pupulus finding in gene bank with ClustalW ver.2.Results showed that P. caspica in all populations withthe same sequence of 339 nucleotide is different with P.nigra (281 nucleotide), P. tremuloides (224 nucleotide),P. nigra (228 nucleotide) that are in gene bank. As inall populations samples P.caspica were observed 112nucleotides substitutions, 78 insertions and 27 gapsthat were differed from other sequences. Based ourfunding, recommend that trnL-F marker can be used asa molecular marker for identification propos.کلیدواژه ها
Populus caspica, Populus nigra, Choloroplast DNA, trnL-F, PCRمقالات مرتبط جدید
- استفاده از جلبک ها در تصفیه پساب فاضلاب: بررسی روش ها، چالش ها و کاربردها
- Isolation and Identification of Effective Probiotics on Staphylococcusepidermidis Strains Isolated from Urine Sample
- اولین گزارش از انگل های خونی میکروسکوپی در وزغ تالشی(Bufo eichwaldi)در شمال ایران
- "Computational Analysis of Isoliquiritigenin as a Potential MAPK۳ Inhibitorin Rectal Cancer: Integrating Transcriptomics, Molecular Docking, andDynamics Simulations"
- “Targeting MMP۷ in Rectal Cancer: Exploring the Therapeutic Potential ofBerberine through Bioinformatics and Molecular Docking Approaches”
اطلاعات بیشتر در مورد COI
COI مخفف عبارت CIVILICA Object Identifier به معنی شناسه سیویلیکا برای اسناد است. COI کدی است که مطابق محل انتشار، به مقالات کنفرانسها و ژورنالهای داخل کشور به هنگام نمایه سازی بر روی پایگاه استنادی سیویلیکا اختصاص می یابد.
کد COI به مفهوم کد ملی اسناد نمایه شده در سیویلیکا است و کدی یکتا و ثابت است و به همین دلیل همواره قابلیت استناد و پیگیری دارد.