Complete Genome Sequence of Oceanimonas sp. GK1: A Halotolerant Bacterium From Gavkhouni Wetland in Iran

Here we present the whole genome sequence ofOceanimonas sp.GK1 which consists of a single circularchromosome with 3514537 bp (CP003171 accessionno.) and two plasmids with 8462 (CP003172 accessionno.) and 4245 bp (CP003173 accession no.) in length.The gram negative rod-shaped Oceanimonas sp. GK1(IBRC-M 10197), isolated from Gavkhouni wetlandin Iran, belongs to the Aeromonadaceae family. It isa motile aerobic bacterium which can tolerate up to12% NaCl and grows at 10- 45°C (optimum 35°C), pH6-10 (optimum 8) and produces high amounts of PHB(poly-β-hydroxybutyrate) under unbalanced growthconditions as carbon and energy reservoir. The wholegenome sequence of this strain is the first report ofcompletely sequenced and annotated genome from theOceanimonas genus.Whole genome sequencing of Oceanimonas sp. GK1was performed on 454 GS-FLX Titanium. In total,93,921,861 bases of 247,884 random reads withaverage read length of 378 nucleotides were obtained.The approximate coverage of Oceanimonas sp.GK1 genome was 30 fold. The sequence reads wereassembled into 72 contigs using Newbler Assemblersoftware v. 2.3. Paired end sequencing resulted in 3scaffolds with 47 gaps. All gaps were closed usingSanger DNA sequencing. The RAST annotation pipelinewas used to annotate the DNA sequence.The complete genome contained 3338 genes with 111structural RNAs, 2516 non-hypothetical protein and 711hypothetical protein coding sequences. The GC contentof the complete genome was 61.1%.The Oceanimonas sp. GK1 genome has the β-ketoacyl-CoA thiolase, acetoacetyl-CoA reductase and PHBsynthase encoded genes from the three–step PHBsynthesis pathway. The investigation of genomesequence showed the presence of enzymes involvedin phenol degradation and ß-ketoadipate pathwaysuch as catechol 1,2- dioxygenase, 3-oxoadipate CoAtransferase(Glutaconate CoA-transferase), muconatecycloisomerase (EC 5.5.1.1) and muconolactoneisomerase (EC 5.3.3.4).

کلیدواژه ها:

Oceanimonas sp. GK1 ، Genome sequencing ، Annotation ، PHB